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Opiate Urine Tests and Process

The One Step Opiate Drug Test is a device intended to detect any of the addictive narcotic pain relieving opiate drugs in urine. An opiate is any natural or synthetic drug that has morphine like pharmacological actions. The opiates include drugs such as morphine. heroin, codeine, nalorphine, and meperidine. Test results obtained by this device are used in the diagnosis and treatment of opiate use. Also, see the hair follicle drug test for expanded opiates.

Opiate Urine based screening tests for drugs of abuse range from complex analytical procedures to simple immunoassay tests. The sensitivity and rapidity of immunoassays have made them the most accepted method of preliminary screening for drugs of abuse in urine. This allows the laboratory to eliminate the large number of negative specimens and focus on the smaller number of initially positive specimens.

A test strip is inserted into a sample cup containing the urine specimen. The urine then migrates up the strip by phoresis. As it migrates it mixes with labeled antibody dye mixture. When opiate or opiate metabolites are present in the urine sample at a concentration at or above 300 ng/ml (the detection sensitivity of the test), antibody dye conjugate binds to free drug forming a complex which competes with immobile antigen conjugate in the test region (T) of the device. This prevents development of a distinct rose-pink band. This indicates a potentially positive sample. 

When sample opiate/opiate metabolites levels are below 300 ng/ml, unbound antibody conjugate binds to immobilized antigen conjugate in the test region (T) of the device. This produces a rose-pink colored band, indicating a negative result. In either case, an identical color band is produced in the control region (C) by a separate antibody/antigen reaction. This band serves as a built-in quality control device which demonstrates antibody recognition and reactivity as well as confirms that the opiate urine testis complete.

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